Investigation and Application Progress of Vero Cell Serum-free Culture
نویسندگان
چکیده
Serum-free culture is now becoming the general trend of biological production. Its application in Vero cell culture is significant. In the present paper, we reviewed factors affecting Vero cell serum-free culture and several culture techniques. In light of these reviews, we outlined its extensive application prospect.
منابع مشابه
Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System
Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...
متن کاملمقایسهی چهار نوع مختلف حامل برای رشد سلول Vero و تکثیر ویروس هاری برای تولید واکسن هاری انسانی
Background and Objectives: In this study, capability of Vero cells for growth on FibraCel disks were compared on 3 kinds of microcarriers including Cytodex-1, Cutodex-3, and Sigma Solohill in 500 ml Spinner flasks in both serum contained medium (DMEM+10% Fetal Calf Serum, FCS) and serum-free medium (VP-SFM). The propagation of fixed PV (Pasteur Virus) strain of rabies in Vero cells, for product...
متن کاملApplication of a serum-free medium for the growth of Vero cells and the production of reovirus.
Two strains of reovirus (serotype 1 Lang/TIL and serotype 3 Dearing/T3D) were propagated in Vero cells grown in stationary or agitated cultures in a serum-free medium, M-VSFM. Solid microcarriers (Cytodex-1) were used to support cell growth in agitated cultures with a normal doubling time of 25 h. Cell yields of 1 x 10(6) cells/mL were obtained from an inoculum of 2 x 10(5) cells/mL in 4 days i...
متن کاملUse of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells.
Neospora caninum and Toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. In vitro cultivation of the protozoans using Vero cells is usually performed in order to produce antigenic materials. Quantitative and qualitative comparisons of Vero cells grown in RPMI medium supplemented either with foetal calf serum (FCS), horse serum (HS) or a specific se...
متن کاملCell fasting: Cellular response and application of serum starvation
Humans suffer transient or persistent starvation due to a lack of food intake, either because of fasting, voluntary dieting, or due to the scarcity of available food. At the cellular level it is possible to possess pathological starvation during ischemia and solid tumors. Blood provides many nutrients to our cells, and researchers provide these nutrients to cells in culture in the form of en...
متن کامل